mouse anti β integrin myospheroid Search Results


rabbit anti β galactosidase  (Valiant Co Ltd)
96
Valiant Co Ltd rabbit anti β galactosidase
Mys is required for interfollicular stalk formation. (A) A wild-type ovariole expressing Mys-GFP (green) from a genomic BAC transgene. Mys is enriched on the basal side of all pre-follicle cells in the germarium (bracket) and in the forming and mature interfollicular stalks (yellow arrowheads and white arrowhead, respectively) ( n =4). (B) A section of ovariole stained for Lgl (red), DNA (blue) <t>and</t> <t>β-galactosidase</t> (green and B′) showing the pattern of UAS-LacZ expression under the control of the 24B-Gal4 driver. 24B-Gal4 drives expression in the interfollicular stalk cells only (arrowhead and arrows) ( n =6). (C) A stage 4 egg chamber stained for Dlg (white) and DNA (blue) containing mys XG43 clones marked by the loss of RFP (red). 24B-Gal4 was used to drive expression of UAS-FLP to generate mys XG43 clones specifically in the interfollicular stalks. The presence of mys mutant cells disrupts the organisation of the interfollicular stalks ( n =14/15). (D) A wild-type stage 4 egg chamber stained for F-actin (white) and DNA (blue) showing the fully formed stalks that separate it from the adjacent younger and older egg chambers ( n =5). (E-J′) For these experiments, mys XG43 clones [marked by the absence of RFP (red)] were induced in interfollicular stalks by the expression UAS-FLP under the control of 24B-Gal4. (E,E′) A disorganised stalk containing mys clones stained for F-actin (green), DNA (blue) and Lamin C (white in E′). Lamin C is expressed in both mutant and wild-type cells in disrupted interfollicular stalks (E) ( n =8). (F,F′) A disorganised stalk containing mys clones stained for Eya (green) and DNA (blue). Eya is turned off normally in both the mutant and wild-type stalk cells ( n =7). (G-I′) Mutant interfollicular stalk cells are round in appearance ( n =33) compared with the stalk made from all wild-type cells (I) ( n =4). Wild-type cells in disrupted interfollicular stalks range from rounded (white asterisks in G,G′) ( n =18/51) to wild type (white asterisks in H,H′) ( n =33/51). Dlg only localises to the regions where neighbouring interfollicular stalk cells contact each other in disrupted interfollicular stalks (arrowheads G′ and H′) ( n =18/18) as in interfollicular stalks containing only wild-type cells (I′) ( n =8/8). (J) A disorganised interfollicular stalk stained for aPKC (green) and Dlg (white). (J′) A z -projection of 16 planes taken 1 μm apart of the stalk in J. This shows that aPKC is not present in mature stalks containing mys XG43 mutant cells ( n =11). (K-L′) Regions of ovarioles stained for DNA (blue) and β-galactosidase (green) containing hs-FLP-induced mys XG43 clones marked by the loss of RFP (red). The interfollicular stalk cells are marked by β-galactosidase (green) expressed from UAS-LacZ under the control of 24B-Gal4. The disorganised region caused by mys XG43 clones at the terminus of the stage 9 egg chamber in the boxed area in K (enlarged in K′) does not contain cells expressing the β-galactosidase stalk marker, which lie only in a malformed stalk posteriorly (K′) ( n =4). (L,L′) The stalk cells lie at the posterior of the region between the two younger egg chambers ( lacZ -positive cells, green). The black dashed line identifies cells that could potentially contribute to the disorganised region at the terminus of the egg chamber later in development, like that seen in K ( n =4). Scale bars: 10 μm.
Rabbit Anti β Galactosidase, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cell signaling technology 9578  (Bio-Rad)
95
Bio-Rad cell signaling technology 9578
Mys is required for interfollicular stalk formation. (A) A wild-type ovariole expressing Mys-GFP (green) from a genomic BAC transgene. Mys is enriched on the basal side of all pre-follicle cells in the germarium (bracket) and in the forming and mature interfollicular stalks (yellow arrowheads and white arrowhead, respectively) ( n =4). (B) A section of ovariole stained for Lgl (red), DNA (blue) <t>and</t> <t>β-galactosidase</t> (green and B′) showing the pattern of UAS-LacZ expression under the control of the 24B-Gal4 driver. 24B-Gal4 drives expression in the interfollicular stalk cells only (arrowhead and arrows) ( n =6). (C) A stage 4 egg chamber stained for Dlg (white) and DNA (blue) containing mys XG43 clones marked by the loss of RFP (red). 24B-Gal4 was used to drive expression of UAS-FLP to generate mys XG43 clones specifically in the interfollicular stalks. The presence of mys mutant cells disrupts the organisation of the interfollicular stalks ( n =14/15). (D) A wild-type stage 4 egg chamber stained for F-actin (white) and DNA (blue) showing the fully formed stalks that separate it from the adjacent younger and older egg chambers ( n =5). (E-J′) For these experiments, mys XG43 clones [marked by the absence of RFP (red)] were induced in interfollicular stalks by the expression UAS-FLP under the control of 24B-Gal4. (E,E′) A disorganised stalk containing mys clones stained for F-actin (green), DNA (blue) and Lamin C (white in E′). Lamin C is expressed in both mutant and wild-type cells in disrupted interfollicular stalks (E) ( n =8). (F,F′) A disorganised stalk containing mys clones stained for Eya (green) and DNA (blue). Eya is turned off normally in both the mutant and wild-type stalk cells ( n =7). (G-I′) Mutant interfollicular stalk cells are round in appearance ( n =33) compared with the stalk made from all wild-type cells (I) ( n =4). Wild-type cells in disrupted interfollicular stalks range from rounded (white asterisks in G,G′) ( n =18/51) to wild type (white asterisks in H,H′) ( n =33/51). Dlg only localises to the regions where neighbouring interfollicular stalk cells contact each other in disrupted interfollicular stalks (arrowheads G′ and H′) ( n =18/18) as in interfollicular stalks containing only wild-type cells (I′) ( n =8/8). (J) A disorganised interfollicular stalk stained for aPKC (green) and Dlg (white). (J′) A z -projection of 16 planes taken 1 μm apart of the stalk in J. This shows that aPKC is not present in mature stalks containing mys XG43 mutant cells ( n =11). (K-L′) Regions of ovarioles stained for DNA (blue) and β-galactosidase (green) containing hs-FLP-induced mys XG43 clones marked by the loss of RFP (red). The interfollicular stalk cells are marked by β-galactosidase (green) expressed from UAS-LacZ under the control of 24B-Gal4. The disorganised region caused by mys XG43 clones at the terminus of the stage 9 egg chamber in the boxed area in K (enlarged in K′) does not contain cells expressing the β-galactosidase stalk marker, which lie only in a malformed stalk posteriorly (K′) ( n =4). (L,L′) The stalk cells lie at the posterior of the region between the two younger egg chambers ( lacZ -positive cells, green). The black dashed line identifies cells that could potentially contribute to the disorganised region at the terminus of the egg chamber later in development, like that seen in K ( n =4). Scale bars: 10 μm.
Cell Signaling Technology 9578, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti cleaved death caspase 1 dcp 1  (Cell Signaling Technology Inc)
97
Cell Signaling Technology Inc rabbit anti cleaved death caspase 1 dcp 1
Mys is required for interfollicular stalk formation. (A) A wild-type ovariole expressing Mys-GFP (green) from a genomic BAC transgene. Mys is enriched on the basal side of all pre-follicle cells in the germarium (bracket) and in the forming and mature interfollicular stalks (yellow arrowheads and white arrowhead, respectively) ( n =4). (B) A section of ovariole stained for Lgl (red), DNA (blue) <t>and</t> <t>β-galactosidase</t> (green and B′) showing the pattern of UAS-LacZ expression under the control of the 24B-Gal4 driver. 24B-Gal4 drives expression in the interfollicular stalk cells only (arrowhead and arrows) ( n =6). (C) A stage 4 egg chamber stained for Dlg (white) and DNA (blue) containing mys XG43 clones marked by the loss of RFP (red). 24B-Gal4 was used to drive expression of UAS-FLP to generate mys XG43 clones specifically in the interfollicular stalks. The presence of mys mutant cells disrupts the organisation of the interfollicular stalks ( n =14/15). (D) A wild-type stage 4 egg chamber stained for F-actin (white) and DNA (blue) showing the fully formed stalks that separate it from the adjacent younger and older egg chambers ( n =5). (E-J′) For these experiments, mys XG43 clones [marked by the absence of RFP (red)] were induced in interfollicular stalks by the expression UAS-FLP under the control of 24B-Gal4. (E,E′) A disorganised stalk containing mys clones stained for F-actin (green), DNA (blue) and Lamin C (white in E′). Lamin C is expressed in both mutant and wild-type cells in disrupted interfollicular stalks (E) ( n =8). (F,F′) A disorganised stalk containing mys clones stained for Eya (green) and DNA (blue). Eya is turned off normally in both the mutant and wild-type stalk cells ( n =7). (G-I′) Mutant interfollicular stalk cells are round in appearance ( n =33) compared with the stalk made from all wild-type cells (I) ( n =4). Wild-type cells in disrupted interfollicular stalks range from rounded (white asterisks in G,G′) ( n =18/51) to wild type (white asterisks in H,H′) ( n =33/51). Dlg only localises to the regions where neighbouring interfollicular stalk cells contact each other in disrupted interfollicular stalks (arrowheads G′ and H′) ( n =18/18) as in interfollicular stalks containing only wild-type cells (I′) ( n =8/8). (J) A disorganised interfollicular stalk stained for aPKC (green) and Dlg (white). (J′) A z -projection of 16 planes taken 1 μm apart of the stalk in J. This shows that aPKC is not present in mature stalks containing mys XG43 mutant cells ( n =11). (K-L′) Regions of ovarioles stained for DNA (blue) and β-galactosidase (green) containing hs-FLP-induced mys XG43 clones marked by the loss of RFP (red). The interfollicular stalk cells are marked by β-galactosidase (green) expressed from UAS-LacZ under the control of 24B-Gal4. The disorganised region caused by mys XG43 clones at the terminus of the stage 9 egg chamber in the boxed area in K (enlarged in K′) does not contain cells expressing the β-galactosidase stalk marker, which lie only in a malformed stalk posteriorly (K′) ( n =4). (L,L′) The stalk cells lie at the posterior of the region between the two younger egg chambers ( lacZ -positive cells, green). The black dashed line identifies cells that could potentially contribute to the disorganised region at the terminus of the egg chamber later in development, like that seen in K ( n =4). Scale bars: 10 μm.
Rabbit Anti Cleaved Death Caspase 1 Dcp 1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Mys is required for interfollicular stalk formation. (A) A wild-type ovariole expressing Mys-GFP (green) from a genomic BAC transgene. Mys is enriched on the basal side of all pre-follicle cells in the germarium (bracket) and in the forming and mature interfollicular stalks (yellow arrowheads and white arrowhead, respectively) ( n =4). (B) A section of ovariole stained for Lgl (red), DNA (blue) and β-galactosidase (green and B′) showing the pattern of UAS-LacZ expression under the control of the 24B-Gal4 driver. 24B-Gal4 drives expression in the interfollicular stalk cells only (arrowhead and arrows) ( n =6). (C) A stage 4 egg chamber stained for Dlg (white) and DNA (blue) containing mys XG43 clones marked by the loss of RFP (red). 24B-Gal4 was used to drive expression of UAS-FLP to generate mys XG43 clones specifically in the interfollicular stalks. The presence of mys mutant cells disrupts the organisation of the interfollicular stalks ( n =14/15). (D) A wild-type stage 4 egg chamber stained for F-actin (white) and DNA (blue) showing the fully formed stalks that separate it from the adjacent younger and older egg chambers ( n =5). (E-J′) For these experiments, mys XG43 clones [marked by the absence of RFP (red)] were induced in interfollicular stalks by the expression UAS-FLP under the control of 24B-Gal4. (E,E′) A disorganised stalk containing mys clones stained for F-actin (green), DNA (blue) and Lamin C (white in E′). Lamin C is expressed in both mutant and wild-type cells in disrupted interfollicular stalks (E) ( n =8). (F,F′) A disorganised stalk containing mys clones stained for Eya (green) and DNA (blue). Eya is turned off normally in both the mutant and wild-type stalk cells ( n =7). (G-I′) Mutant interfollicular stalk cells are round in appearance ( n =33) compared with the stalk made from all wild-type cells (I) ( n =4). Wild-type cells in disrupted interfollicular stalks range from rounded (white asterisks in G,G′) ( n =18/51) to wild type (white asterisks in H,H′) ( n =33/51). Dlg only localises to the regions where neighbouring interfollicular stalk cells contact each other in disrupted interfollicular stalks (arrowheads G′ and H′) ( n =18/18) as in interfollicular stalks containing only wild-type cells (I′) ( n =8/8). (J) A disorganised interfollicular stalk stained for aPKC (green) and Dlg (white). (J′) A z -projection of 16 planes taken 1 μm apart of the stalk in J. This shows that aPKC is not present in mature stalks containing mys XG43 mutant cells ( n =11). (K-L′) Regions of ovarioles stained for DNA (blue) and β-galactosidase (green) containing hs-FLP-induced mys XG43 clones marked by the loss of RFP (red). The interfollicular stalk cells are marked by β-galactosidase (green) expressed from UAS-LacZ under the control of 24B-Gal4. The disorganised region caused by mys XG43 clones at the terminus of the stage 9 egg chamber in the boxed area in K (enlarged in K′) does not contain cells expressing the β-galactosidase stalk marker, which lie only in a malformed stalk posteriorly (K′) ( n =4). (L,L′) The stalk cells lie at the posterior of the region between the two younger egg chambers ( lacZ -positive cells, green). The black dashed line identifies cells that could potentially contribute to the disorganised region at the terminus of the egg chamber later in development, like that seen in K ( n =4). Scale bars: 10 μm.

Journal: Development (Cambridge, England)

Article Title: The role of integrins in Drosophila egg chamber morphogenesis

doi: 10.1242/dev.182774

Figure Lengend Snippet: Mys is required for interfollicular stalk formation. (A) A wild-type ovariole expressing Mys-GFP (green) from a genomic BAC transgene. Mys is enriched on the basal side of all pre-follicle cells in the germarium (bracket) and in the forming and mature interfollicular stalks (yellow arrowheads and white arrowhead, respectively) ( n =4). (B) A section of ovariole stained for Lgl (red), DNA (blue) and β-galactosidase (green and B′) showing the pattern of UAS-LacZ expression under the control of the 24B-Gal4 driver. 24B-Gal4 drives expression in the interfollicular stalk cells only (arrowhead and arrows) ( n =6). (C) A stage 4 egg chamber stained for Dlg (white) and DNA (blue) containing mys XG43 clones marked by the loss of RFP (red). 24B-Gal4 was used to drive expression of UAS-FLP to generate mys XG43 clones specifically in the interfollicular stalks. The presence of mys mutant cells disrupts the organisation of the interfollicular stalks ( n =14/15). (D) A wild-type stage 4 egg chamber stained for F-actin (white) and DNA (blue) showing the fully formed stalks that separate it from the adjacent younger and older egg chambers ( n =5). (E-J′) For these experiments, mys XG43 clones [marked by the absence of RFP (red)] were induced in interfollicular stalks by the expression UAS-FLP under the control of 24B-Gal4. (E,E′) A disorganised stalk containing mys clones stained for F-actin (green), DNA (blue) and Lamin C (white in E′). Lamin C is expressed in both mutant and wild-type cells in disrupted interfollicular stalks (E) ( n =8). (F,F′) A disorganised stalk containing mys clones stained for Eya (green) and DNA (blue). Eya is turned off normally in both the mutant and wild-type stalk cells ( n =7). (G-I′) Mutant interfollicular stalk cells are round in appearance ( n =33) compared with the stalk made from all wild-type cells (I) ( n =4). Wild-type cells in disrupted interfollicular stalks range from rounded (white asterisks in G,G′) ( n =18/51) to wild type (white asterisks in H,H′) ( n =33/51). Dlg only localises to the regions where neighbouring interfollicular stalk cells contact each other in disrupted interfollicular stalks (arrowheads G′ and H′) ( n =18/18) as in interfollicular stalks containing only wild-type cells (I′) ( n =8/8). (J) A disorganised interfollicular stalk stained for aPKC (green) and Dlg (white). (J′) A z -projection of 16 planes taken 1 μm apart of the stalk in J. This shows that aPKC is not present in mature stalks containing mys XG43 mutant cells ( n =11). (K-L′) Regions of ovarioles stained for DNA (blue) and β-galactosidase (green) containing hs-FLP-induced mys XG43 clones marked by the loss of RFP (red). The interfollicular stalk cells are marked by β-galactosidase (green) expressed from UAS-LacZ under the control of 24B-Gal4. The disorganised region caused by mys XG43 clones at the terminus of the stage 9 egg chamber in the boxed area in K (enlarged in K′) does not contain cells expressing the β-galactosidase stalk marker, which lie only in a malformed stalk posteriorly (K′) ( n =4). (L,L′) The stalk cells lie at the posterior of the region between the two younger egg chambers ( lacZ -positive cells, green). The black dashed line identifies cells that could potentially contribute to the disorganised region at the terminus of the egg chamber later in development, like that seen in K ( n =4). Scale bars: 10 μm.

Article Snippet: The following antibodies were used in this study: mouse anti-Fasciclin II (1D4), mouse anti-Discs large (4F3), rabbit anti-Myospheroid (CF.6G11), mouse anti-Lamin C (LC28.26), mouse anti-Eyes absent (eya10HB), mouse anti-Armadillo (N2 7A1) (all Developmental Studies Hybridoma Bank), rabbit anti-aPKC (Sigma-Aldrich, P0713), rabbit anti-β-galactosidase (MP Biomedicals, 0855976), rabbit anti-Lethal giant larvae (Santa Cruz Biotechnology, sc-98260) and mouse anti-Fasciclin III (gift from C. Goodman, UC Berkeley, USA).

Techniques: Expressing, Staining, Clone Assay, Mutagenesis, Marker